Departmental Instructions

 

Department of Botany

 Laboratory Precautions:

• Handle plant material as per instructions.

• Use sharp instruments carefully.

• Dissection box is compulsory.

• Handbook and journal is compulsory.

• Come five minutes before your practical schedule. 

• Don’t eat any plant material or food in laboratory.

• Handle microscope as per instructions.

• Put your bag aside during practical.

• Don't waste chemicals and plant material. 

• Clean equipment  before and after completion of practical.

• Handle chemical, stains and regent carefully.

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• Follow the warning during using hazard chemicals.

• Wash your hands after practical.

• Know the emergency and fire procedures.

• Discard cracked or broken glass in a “ broken glass container.”

DO's 

1. Always wear lab coat, shoes in the laboratory. Every student must have their weight box, a napkin etc. 2. Maintain separate record book for each subject. 3. Keep your belongings at the place allotted for the same. 4. Maintain silence, order, cleanliness and discipline in the laboratory. 5. Work at the place allotted to you or specially used for certain operations. 6. Keep the working table clean. 7. Handle the laboratory equipment's, glassware and chemical with great care. 8. Use only required quantities of material and apparatus of essential size. 9. Perform the test in their proper order. 10. Know the location of eye wash fountain and water shower. 11. Minimise your exposure to organic solvents. 12. The Metal like sodium should be kept under kerosene or liquid paraffin layer in a vessel with a cork stopper. 13. Sodium metal should be cut on dry filter paper. The cut off pieces of sodium should be immediately collected in a vessel containing kerosene or liquid paraffin. 14. Always pour acid into water when diluting and stir slightly. 15. All operations involving poisonous flammable gases and vapours should be carried out in the flame chamber (with exhaust facility) 16. Ladies should avoid wearing saree. If it is there, apron is essential.

 DON’T 

1.Don’t work alone in the laboratory 2. Don’t leave the glass wares unwashed. 3. Don’t take apparatus, chemicals out of lab. 4. Don’t leave any substance in a vessel or bottle without label. 5. Don’t weigh the reagent directly on the balance pan. 6. Don’t throw the cut off pieces of sodium metal in sink or water. Transfer it immediately in its container. 7. Don’t take sodium metal with hands. Use forceps. 8. Don’t panic and run in case of fire. Use the fire extinguishers or sand buckets. Shivaji University, Kolhapur. B.Sc. III Botany CBCS Syllabus implemented from June 2020 Page 25 9. Don’t breathe the vapours of organic solvents. 10. Don’t pour any unused reagent back in its stock bottle. 11. Don’t eat or drink any food in laboratory. 12. Don’t use inflammable solvents like benzene, ether, chloroform, acetone and alcohol around flame. 13. Don’t distill to dryness. 14. Don’t exchange stoppers of flasks and bottles containing different reagents. 15. Don’t leave reagent bottle lying on the table. 16. Don’t disturb the order of reagent bottles in which they are placed. 17. Don’t bring reagent on your working table from the general shelf. 18. Don’t throw burning matchstick into dustbin. 19. Don’t leave the laboratory without permission. 5)

 LABORATORY / FIELD WORK CARE AND SAFETY FOR BOTANY STUDENTS 

1. Unnecessary wastage of plant material during practical should be avoided. 2. During study tour / personal collection, more emphasis be given on study of plants in nature and collection of wild plants should not be carried out. 3. If at all the collection of the plant material in needed, it should be carried out under supervision of concerned teacher. Collection of poisonous plants / poisonous mushrooms should be avoided. 4. Oral intake of unknown plant material, out of curiosity, during practical or collection tour is strictly prohibited. 5. If there is any allergic reaction while handling the plants / plant parts / pollen grains / fungal specimens it should be immediately brought to the notice of the concerned teacher and reported to the registered medical purloiner. 6. Wearing of hand gloves (and mask) is essential while handling poisonous plants / herbarium sheets / toxic and hazardous chemicals / reagents / strong acids / strong alkalis during the experiment should be made with vacuum pipette / auto pipette / burette under the supervision of concerned teacher / lab assistant. 7. Highly inflammable organic solvents (alcohol, acetone etc.) should not be kept in vicinity of spirit lamp. 8. The laboratory safety measures adopted for handling of hazardous chemicals in chemistry practicals should be followed for conducting practicals in plant biochemistry / microbiology. 9. Operational manuals for equipments such or centrifuge, autoclave, spectrophotometer should be followed. Shivaji University, Kolhapur. B.Sc. III Botany CBCS Syllabus implemented from June 2020 Page 26 10. In case of minor injuries, preliminary treatment should be undertaken with the help of first aid kit available in the laboratory. In case of serious injury, concerned teacher should be immediately contacted for consultation to the physician. 11. The instruction report for breeding, experimentation will be submitted in a week period. (Which are laid down by Ministry of Social Justice & Empowerment and Ministry of Environment and Forests, Govt. of India). 

Course outcomes:

 1. Students are acquainted with basic as well as recent knowledge in the field of molecular biology, biotechnology and bioinformatics 

2. Acquiring the basic procedure in the field of microbiology and plant pathology.

 3. To develop skills in of horticulture including nursery, landscaping, gardening, floriculture and polomology

 4. Students will be able to demonstrate their knowledge, skills and attributes to be successful contributing members o the horticulture profession. 

5. Acquaint the student with the comprehensive knowledge in the bio fertilizers, herbal drug technology and paleobotany 6. Students will able to demonstrate their understanding of relevant course theories and concepts Students able to Mendelian and Neo-mendelian genetics

 7. Students become familiar with the Organic manures, Herbal Medicines, Herbal cosmetology and Pharmacognosy. 

8. Understand the methods of Plant Biotechnology, Protoplast culture and Recombinant DNA Technology.

 9. Acquainted the scope of paleobotany in the present scenario and understand the fossil genera

 10. Aware about the Spices, Beverages and Fibers, Cereals, Legumes and Oils.

 11. Understand the of carbohydrates, lipids, proteins 

12. Understand the techniques of plant breeding 

13. Understand the techniques of mushroom cultivation. 

14. Acquainted the techniques of micrometry, chromatography and other laboratory techniques used in the field of life science.